| How
to handle lables with the LT Colony Counter
Labels on petridishes are generally a sign for automatation
already started. If the label contains a barcode this could be used
for naming the plate within the LT counter system too. Thus typing
of the name of the plate, a time consuming part of sample handling
and source of faults can be avoided. The LT counter is easily able
to integrate barcode reading as additional option. To provide an
optimised barcode reader some samples of the label with barcode,
technical specification of the barcode (if available) and information
on the nomenclature used should be available from LemnaTec..
But in detail labels can even
provide some problems.
Optimally for image acquisition barcode is to be found
at the side of the petridish as this opens all options for choosing
the best illumination. Placement of the barcodes on the lid of the
petridish is good as long as lid may be removed for image acquisition.
If the barcode is on the lid not to be removed or on the bottom
two different options are available. If the colonies have good contrast
and have darker colour than the background they may be counted in
Backlight after special adaptation of the image processing. For
dark colonies, which may be counted on white background with top
light, a white insert for the top light masks is available. This
makes white labels almost invisible. In this case plates with label
on the lid may be counted up side down while plates with label on
the bottom will be counted in normal position. For pale colonies
on almost clear agar the only option is to use preferably backlight
and to extrapolate the number of colonies in the area of the label,
as they could not be counted (except for labels based on black paper
would be available).
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